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KMID : 0604520130390010031
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Journal of the Society of Cosmetic Scientists of Korea
2013 Volume.39 No. 1 p.31 ~ p.38
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Assessment of Skin Toxicity Using Skin Equivalents Containing Cervi cornus Colla
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Kim Jan-di
Lee Hai-Lan
Jeong Hyo-Soon
Yun Hye-Young
Baek Kwang-Jin
Kwon Nyoun-Soo
Choi Hye-Ryung
Park Kyoung-Chan
Kim Dong-Seok
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Abstract
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To substitute animal test, skin equivalents (SEs) have been developed for skin irritation and corrosion test. Recently, we have developed new SEs containing Cervi cornus Colla (CCC). In the present study, we used the SEs for cutaneous cytotoxicity test. Sodium dodecylsulfate (SDS) or sodium carbonate was applied to the SEs, and the epidermal damage by H&E and immunohistochemical stains was evaluated. Our results showed that SDS or sodium carbonate affected the epidermal part of SEs containing CCC in a dose-dependent manner and decreased the expression of p63. It is concluded that SEs containing CCC could be used for an alternative model of animal test and would be greatly helpful in the development of in vitro irritation and corrosion test.
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KEYWORD
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in vitro irritation test, non-animal test, cutaneous cytotoxicity, human skin equivalents, Cervi cornus Colla
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